Algorithms for multiparameter reconstruction of spectral and luminescence characteristics by the fluorescence saturation method

Considered is the possibility of using fluorescence saturation curves to find the following molecular parameters: (i) the quantum yield of triplet states, (ii) the lifetime of the first excited singlet state $S_1$, and (iii) the absorption cross section for a transition from the ground state to $S_1$ at the wavelength of excitation. Algorithms for a simultaneous determination of two and three parameters have been proposed. Requirements to the accuracy of saturation curve measurements were found by mathematical modeling. The results of an experimental check-up of the proposed solution algorithm for a three-parameter problem are presented for the case of eosine aqueous solution.