Annotation
The pH dependence of the intermolecular interaction coefficient of pepsin in an aqueous solution is obtained by using photon-correlation spectroscopy. The dependence shows a minimum near the isoelectric point (pH 1.6). The dynamic parameters of pepsin macromolecules in solutions containing various metal ions, including potassium, cobalt, lead, cesium, and rubidium are studied. It is shown that the addition of these ions causes a decrease of the translational diffusion coefficients. It is found that an increase in temperature leads to the structural transition of a pure solution of pepsin in water. The activation energy of this transition is calculated using the Arrhenius law.
Received: 2011 June 17
Approved: 2012 March 21
PACS:
78.35.+c Brillouin and Rayleigh scattering; other light scattering
87.15.-v Biomolecules: structure and physical properties
33.15.-e Properties of molecules
42.62.-b Laser applications
87.15.-v Biomolecules: structure and physical properties
33.15.-e Properties of molecules
42.62.-b Laser applications
© 2016 Publisher M.V.Lomonosov Moscow State University
Authors
Xiaolei Zhang, G.P. Petrova, Yu.M. Petrusevich, I.A. Sergeeva
Faculty of Physics, Moscow State University, Moscow, 119991, Russia
Faculty of Physics, Moscow State University, Moscow, 119991, Russia