Annotation
The binding of biotinylated aptamers to Lon protease, whose structures contain G-quadruplex fragments, with magnetic nanoparticles (MNP) functionalized by streptavidin is investigated. The conditions of the binding of target aptamers to MNP are fulfilled. The resulting complexes are assumed to be used for detection of Lon protease in different biological sources and for constructing of novel biomagnetic nanosensor system of the enzyme immunoassay.
Received: 2016 June 15
Approved: 2017 October 2
PACS:
80.00.00 INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY
70.00.00 CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES
70.00.00 CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES
© 2016 Publisher M.V.Lomonosov Moscow State University
Authors
V.A. Spiridonova$^1$, V.A. Sizov$^2$, E.O. Kuzmenko$^2$, A.V. Melnichuk$^1$, E.A. Oleinichenko$^1$, A.M. Kudzhaev$^3$, T.V. Rotanova$^3$, O.V. Snigirev$^4$
$^1$Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University\
$^2$Faculty of Chemistry, Lomonosov Moscow State University\
$^3$Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS\
$^4$Department of General Nuclear Physics, Faculty of physics, Lomonosov Moscow State University, chair of Physics of Semiconductors.
$^1$Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University\
$^2$Faculty of Chemistry, Lomonosov Moscow State University\
$^3$Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS\
$^4$Department of General Nuclear Physics, Faculty of physics, Lomonosov Moscow State University, chair of Physics of Semiconductors.