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ATP-dependent complexes of the LON-protease and DNA aptamers with G-quadruplex as a model for developing a nanosensor biomagnetic system immunoassay

V.A. Spiridonova$^1$, V.A. Sizov$^2$, E.O. Kuzmenko$^2$, A.V. Melnichuk$^1$, E.A. Oleinichenko$^1$, A.M. Kudzhaev$^3$, T.V. Rotanova$^3$, O.V. Snigirev$^4$

Moscow University Physics Bulletin 2017. 72. N 4. P. 376

  • Article
Annotation

The binding of biotinylated aptamers to Lon protease, whose structures contain G-quadruplex fragments, with magnetic nanoparticles (MNP) functionalized by streptavidin is investigated. The conditions of the binding of target aptamers to MNP are fulfilled. The resulting complexes are assumed to be used for detection of Lon protease in different biological sources and for constructing of novel biomagnetic nanosensor system of the enzyme immunoassay.

Received: 2016 June 15
Approved: 2017 October 2
PACS:
80.00.00 INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY
70.00.00 CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES
Rissian citation:
В.А. Спиридонова, В.А. Сизов, Е.О. Кузьменко, А.В. Мельничук, Е.А. Олейниченко, А.М. Куджаев, Т.В. Ротанова, О.В. Снигирев.
Комплексы АТР-зависимой LON-протеазы и ДНК аптамеров с G-квадруплексной структурой как модель для разработки наносенсорной биомагнитной системы безразделительного иммунного анализа.
Вестн. Моск. ун-та. Сер. 3. Физ. Астрон. 2017. № 4. С. 34.
Authors
V.A. Spiridonova$^1$, V.A. Sizov$^2$, E.O. Kuzmenko$^2$, A.V. Melnichuk$^1$, E.A. Oleinichenko$^1$, A.M. Kudzhaev$^3$, T.V. Rotanova$^3$, O.V. Snigirev$^4$
$^1$Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University
$^2$Faculty of Chemistry, Lomonosov Moscow State University
$^3$Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS
$^4$Department of General Nuclear Physics, Faculty of physics, Lomonosov Moscow State University, chair of Physics of Semiconductors.
Issue 4, 2017

Moscow University Physics Bulletin

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