The binding of biotinylated aptamers to Lon protease, whose structures contain G-quadruplex fragments, with magnetic nanoparticles (MNP) functionalized by streptavidin is investigated. The conditions of the binding of target aptamers to MNP are fulfilled. The resulting complexes are assumed to be used for detection of Lon protease in different biological sources and for constructing of novel biomagnetic nanosensor system of the enzyme immunoassay.
70.00.00 CONDENSED MATTER: ELECTRONIC STRUCTURE, ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES
$^1$Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University
$^2$Faculty of Chemistry, Lomonosov Moscow State University
$^3$Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS
$^4$Department of General Nuclear Physics, Faculty of physics, Lomonosov Moscow State University, chair of Physics of Semiconductors.