Annotation
We used aptamers, which are functional equivalents of antibodies, in order to develop a nanosensor immunoassay system based on magnetic nanoparticles and a SQUID magnetometer. Selection was used to obtain DNA aptamers to interleukin-6; their affinity to the target protein was characterized by surface plasmon resonance. It was shown that the biotinylated aptamer binds to magnetic nanoparticles that were functionalized with streptavidin.
Received: 2015 August 19
Approved: 2016 March 11
PACS:
87.83.+a Biomedical applications of nanotechnology
87.15.B- Structure of biomolecules
87.90.+y Other topics in biological and medical physics
87.15.B- Structure of biomolecules
87.90.+y Other topics in biological and medical physics
© 2016 Publisher M.V.Lomonosov Moscow State University
Authors
V.A. Sprirdonova$^1$, T.M. Novikova$^1$, O.V. Snigirev$^2$
$^1$Belozersky Institute of Physicochemical Biology, Moscow State University, Moscow, 119991 Russia
$^2$Department of Physics, Moscow State University, Moscow, 119991 Russia
$^1$Belozersky Institute of Physicochemical Biology, Moscow State University, Moscow, 119991 Russia
$^2$Department of Physics, Moscow State University, Moscow, 119991 Russia
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