Annotation
This paper summarizes the results of research into fluorescence saturation of natural organic complexes. Problems are analyzed that have to be solved in order to make nonlinear fluorimetry (saturation fluorimetry) a fundamentally new method of spectroscopy enabling one to determine molecular photophysical parameters of composite organic compounds and complexes $\textit{in vivo}$ and $\textit{in situ}$. Two approaches are offered for solving inverse problems of saturation fluorimetry. The results of computer and actual experiments are presented that illustrate the possibilities of nonlinear laser fluorimetry in natural organic complexes diagnostics.
© 2016 Publisher M.V.Lomonosov Moscow State University
Authors
V.V. Fadeev
Faculty of Physics, Moscow State University, Leninskie Gory, Moscow, 119992, Russia
Faculty of Physics, Moscow State University, Leninskie Gory, Moscow, 119992, Russia