The polarized fluorescence of the Rose Bengal fluorescent nanomarker in HSA solutions was investigated and parameters of its rotational diffusion were calculated. The increase in the degree of fluorescence polarization, rotational relaxation time, and the effective hydrodynamic radius of Rose Bengal, as well as the decrease of the rotational diffusion coefficient in HSA solutions, were found. The effects of the electronegativity of atoms in the structure of the nanomarker on the parameters of its rotational diffusion were established based on comparison of Rose Bengal with other nanomarkers of the homologous family.
87.15.Vv Diffusion
Faculty of Physics, Moscow State University, Moscow, Russia
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